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Biology Program

Dr. Dubytska's Website

Lidiya Dubytska, MS, Ph.D.
Associate Professor 
Biology
E-mail: Lidiya_Dubytska@subr.eduor Dubytska@yahoo.com

Biography

Dr. Lidiya Dubytska is an Associate Professor of Biology at the Southern University-Baton Rouge (SUBR).

Dr. Dubytska received an MS degree from the Department of Biochemistry, Ivan Franko State University of L'viv, Ukraine. The main emphasis of her research was to study the influence of transforming growth factor beta (TGF-beta) in the contraction of collagen gels by the fibroblast line, NIH-3T3.  She completed her Ph.D. in 2003 from the Institute of Cell Biology and Genetic Engineering of the National Academy of Science of Ukraine, Kiev, Ukraine in Molecular Genetics. Her research focused on the construction of a strain of Streptomyces peucetius subsp. Caesius ACC27952-2 that produced anticancer drags daunorubicin and doxorubicin.  During this time, her work resulted in nine peer-reviewed original research articles and four presentations at the Symposia. She received prize of National Academy Science of Ukraine for the best presentation at the International Scientific Meeting held at Institute of Molecular Biology and Genetics in 2002.  She also patented the Method of obtaining strains of Streptomyces peucetius subsp. caesius ATCC 27952 with elevated synthesis of doxorubicin and capability of converting daunorubicin to doxorubicin. Publ. 2002 in Ukraine: Pat. Ukraine. 50047 entitled, “Pat. Ukraine. 50047

After completing her Ph.D she was accepted as an Assistant Professor to teach molecular biology and parasitology to medical students in Ivano-Frankivsk State Medical Academy (Ivano-Frankivsk, Ukraine). In 2003, she accepted a Postdoctoral position at the New York Medical College, Valhalla, NY. And was soon promoted to Postdoctoral Research Associate at New York Medical College. She was also awarded with permanent resident cart on National interest sponsored by NYMC. During this time, she published 2 prereview articles.

For 6 years, she worked as a Postdoctoral Research Associate first and then as Research Assistant Professor. Her research focused on bacterial genome and plasmids sequence, characterization of atypical plasmids of E. ictaluri from Asia, identification and characteristic of T3SS effectors of E.ictaluri, modulation of host cell organelle trafficking by E. ictaluri, detection of host cell protein targets for T3SS effectors and the innate immune response to E. ictaluri. During this time, she was able to publish 6 peer-reviewed original articles and eleven abstracts. 2 complete bacterial genomes and multiple plasmids was completed, annotated and submitted to a database. During this time, she also received a CEIDER support fund grant for using department facilities and a local LSU grant (Proposal for LSU/SVM USDA 1433 funds) as a Co-PI.

Dr. Dubytska joined SUBR in 2017 as an Associate Professor and create new direction in SUBR research related to microbiology, pathogen host interaction and host immune response. During first year of her appointment she resided Pilot LBRN grant. She published 2 peer-reviewed original articles and present her work on 2 scientific meetings. Her student was placed second in biology undergraduate poster presentation.   In addition, she thought graduate and undergraduate level students in two prominent universities in Ukraine: Ivan Franko L’viv State University and Ivan-Frankivsk State Medical Academy. And doctorate students at LSU. In total she punished over 50 original research articles.

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Area of Interest

Virulence is a complex trait that is usually multifactorial. Evolutionary selected systems of virulence gene regulation allow coordinated regulation of gene expression based on the temporal and special requirements of host niches. Global regulation of virulence genes is a common strategy of bacterial pathogens to overcome the complexity of innate and acquire host defenses. Coordinated expression of virulence genes can be achieved through multiple mechanisms. In addition to clustering genes in operons that are transcribed into policistronic messages from a single promoter, such as pathogenicity islands of Type three secretion system (T3SS) in many bacteria, there are several other global regulatory mechanisms that lead to coordinated regulation of expression of independent virulent genes and operons. These include alternative sigma factors, chromosomally encoded proteins like  histone-like H-NS that bind to DNA and regulate gene expression, regulatory cascades, two-component systems to transduce signals from the bacterial envelop, quorum sensing, and the stringent response. 

My overarching research interest is in the mechanisms associated with virulence of bacterial pathogens and for the development of strategies for protection and treatments against these pathogens.  Deeper understanding of the molecular mechanisms of virulence can be effectively converted to practical use for disease control. A better understanding of the role of bacterial virulence genes and toxins that are delivered to host cells and knowledge of host immune/inflammatory response is critical for development of better intervention strategies. Finally, an in-depth knowledge of bacterial pathogenesis will allow intelligent selection of a specific gene or a combination of genes as candidates for mutagenesis for vaccine development.

Honors

2008                 Permanent resident card on national interest from NYMC

Research awards:

  • Award for the best presented scientific work.

National Academy Science of Ukraine. International Scientific Meeting at the Institute of Molecular Biology and Genetic, Kiev, Ukraine.  

 2019 Demetrius McAtee, Dr. Dubytska's undergraduate advisee, obtained second place in the Biology undergraduate poster competition at the 76th Annual Joint Conference of BKX and NIS (March 28-30, 2019, Atlanta, GA). 

Second place BKX 2019

Demetrius McAtee at the 76th BKX and NIS poster competition

Patent:

  • Ukraine. 50047. Method of obtaining strains of Streptomyces peucetius subsp. caesius ATCC 27952 with elevated synthesis of doxorubicin and capability of converting daunorubicin to doxorubicin. Publ. 2002.

Selected Publications 

  1. Dubytska LP Borrelia burgdorferi 297 bmpA Encode the mRNA that Contains ORF for a Leader Peptide that Regulates bmpA Gene Expression. 2019. J Biomed Res Rev Vol: 2, Issu: 1 (34-44).
  2. Dubytska L.P. and Thune R.L. 2018. Type Three Secretion System Effector EseN is a Phosphothreonine Lyase That Inactivates ERK1/2. 2018. Dis Aquat Organ. Sep 10; 130:117-129.
  3. Dubytska LP, Rogge ML, Thune RL. Identification and Characterization of Putative Translocated Effector Proteins of the Edwardsiella ictaluri Type III Secretion System. mSphere 1.
  4. Ucko M, Colorni A, Dubytska L.P, Thune R. L. 2015. Edwardsiella marina (sp. nova) carrying a locus of enterocyte effacement (LEE): a new pathogen in cultured groupers. Submitted to Diseases of Aquatic Organisms.
  5. Reichley S.R., Waldbieser G.C., Ucko M., Colorni A., Dubytska L.D., Thune R.L., Lawrence M. L., and Griffin M.J. 2015. Complete genome sequence of an Edwardsiella piscicida-like species isolated from diseased grouper in Israel. Genom Announc 3(4):e00829-15.doi:10.1128/genomeA00829-15.
  6. Baumgartner A.*, Dubytska L.P.*,  Roggeand M. L., Mottram P.J. and Thune R. L. 2014. Modulation of vacuolar pH is required for replication of Edwardsiella ictaluri in channel catfish macrophages.Infect Immun. Jun; 82(6):2329-36. (*Shared first authorship).
  7. Rogge L.*, Dubytska L.P.*,  Jung T.S., Wiles J.,  Elkamel A.A.,  Rennhoff A.,  Oanh D. T.,  Thune R. L. 2013. Comparison of Vietnamese and US isolates of Edwardsiella ictaluri.  Dis Aquat Organ. Sep 24; 106(1):17-29. (* Shared first authorship).
  8. Dubitckaia P. (Dubytska), Fedorenko V. A. 2002. Effect of glucose on the antibiotic activity and antibiotics resistance of Streptomyces peucetius subsp. caesius ATCC 27952 - 2 and its mutants. Antibiotics and chemotherapy. Vol. 47, N6, P.7-11.
  9. Cabello F. C., Dubytska L., Bryksin A.V., Bugrysheva J.V. and Godfrey H.P. 2006. Genetic Studies of the Borrelia burgdorferi bmp gene family. Molecular biology of Spirochetes IOS Press.
  10. Morozova O.V., Dubytska L.P., IvanovaB., Moreno C.X., et al. 2005. Genetic and physiological characterization of 23 S rRNA and ftsJ mutants of Borrelia burgdorferi isolated by mariner transposition. Gene. P. 63-72.
  11. Dubytska L.P., Fedorenko V.O. 2002. The characterization of Streptomyces peucetius subsp. caesius ATCC 27952 mutants resistant to chloramphenicol. Visnyk L'viv univ. Ser. Biology. 2002. Is 28. P. 1005-113.
  12. Dubitska L.P. (Dubytska). 2001. Construction and selection strain Streptomyces peucetius subsp. caesius production daunorubicin and doxorubicin. Manuscript. The Candidate Thesis for Philosophy Degree by specialty 03.00.15. Genetics. Institute of cell biology and gene enginery, National Academy of Sciences of Ukraine, Kyiv, 2001. 18 - Pages.
  13. Dubitska L.P. (Dubytska). 2002. Construction of the strains Streptomyces peucetius subsp. caesius ATCC 27952 with elevated ability conversion daunorubicin to doxorubicin. Biopolymers and cell. N2 Vol 18. P. 91-95
  14. Dubytska L.P., Fedorenko V.O. 2001. Characterization of mutants of Streptomyces peucetius subsp. caesius ATCC 27952 resistant to antracycline antibiotics. Mikrobiologichny Zhurnal V63 N4. P. 53-61.
  15. Fedorenko V.O., Basiliya L.I., Zavorotna S.A., Kirichenko N.V., Nastasak I.N., Demedchuk J.O, Dubytska L.P., Mazepa A.F., Marina O.V., Pankevych K.O., Ostash O., Luzeckyy A.M., Gromiko O.M., Sambir M.B. 2001. Genetic control of antibiotic biosynthesis ant resistance to antibiotic in Actinomycetes. Genetic and selection in Ukraine on border the millennium. Volume 1. Page:  469-477.  Ukrainian.
  16. Dubytska L.P., Fedorenko V.O. 2001. Way to obtain strain Streptomyces peusetius subsp. caesius with higher biosynthesis of doxorubycin and ability to transformation daunorubicin to doxorubicin. Science – practical conference: Problem invention and rationalization in Ukraine Page 64-68. Ukrainian.
  17. Dubytska L., Zavorotna S., Ostash B. at al. 2000. Characterization of resistance pattern to antibiotics of producers of antitumor antibiotics Streptomyces globisporus 1912, Streptomyces peucetius ATCC 29050 and Streptomyces peucetius subsp. caesius ATCC 27952. Visnyk L'viv Univ. Ser. Biology. Is 25. P. 49-60.
  18. Fedorenko V.O., Basiliya L.I, Pankevych K.O., Dubytska L.P., et al. 2002. Genetic control of anticancer- polyketides biosynthesis by Actinomycetes. Bull. Inst. Rural Microbiol.; 8: 27-31.

Gene bank submissions:

  1. Accession: NC_020295.1 Dubytska L.P. and Thune, R.L. Edwardsiella ictaluri strain 2s plasmid 2s-pEI2, complete sequence.
  2. Accession: NC_020281.1 Dubytska L.P. and Thune, R.L. Edwardsiella ictaluri strain 19s plasmid 19s-pEI3, complete sequence
  3. Accession: NC_020280.1 Dubytska L.P. and Thune, R.L. Edwardsiella ictaluri plasmid pEI3, complete sequence
  4. Accession: NC_020282.1 Dubytska L.P. and Thune, R.L. Edwardsiella ictaluri strain CAF 258 plasmid pEI1, complete sequence
  5. Accession: NC_020279.1 Dubytska L.P. and Thune, R.L. Edwardsiella ictaluri strain 2s plasmid pEI1, complete sequence
  6. Accession: CP011364.1 Dubytska L.P. and Thune, R.L. Edwardsiella sp. EA181011, whole genome shotgun sequence.
  7. Accession: KP234023.1 Thune, R.L and Dubytska L.P. Edwardsiella sp. EA181011, genomic sequence
  8. Accession: NC_023029.1 L Dubytska and R. Thune Francisella noatunensis subsp. orientalis LADL--07-285A, complete genome.

 

Other Experience and Professional Memberships

2014-Present.    Inviting review in Advances in Microbiology (AiM), Scientific Research Publishing

2017-Present.   Inviting review in BMC Microbiology.

2019-Present on Editorial Advisory Board in the journal international medicine

  1. Revision of 15th edition of Benson’s Microbiological Applicaons: Laboratory Manual in General Microbiology by Heidi Smith. McGraw-Hill Educaon.

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